Single-particle imaging by x-ray free-electron lasers—How many snapshots are needed?
نویسندگان
چکیده
منابع مشابه
Toward Single-particle Bioimaging Using X-ray Free-electron Lasers
In this paper we review the recent progress toward single-particle imaging of biological molecules at x-ray free-electron laser (XFEL) facilities. We describe the progression from biological imaging at synchrotrons to imaging at XFELs, discuss recent successes, and point out specific challenges associated with imaging at XFEL facilities.
متن کاملSimple convergent-nozzle aerosol injector for single-particle diffractive imaging with X-ray free-electron lasers
A major challenge in high-resolution x-ray free-electron laser-based coherent diffractive imaging is the development of aerosol injectors that can efficiently deliver particles to the peak intensity of the focused X-ray beam. Here, we consider the use of a simple convergent-orifice nozzle for producing tightly focused beams of particles. Through optical imaging we show that 0.5 μm particles can...
متن کاملSingle particle X-ray diffractive imaging.
In nanotechnology, strategies for the creation and manipulation of nanoparticles in the gas phase are critically important for surface modification and substrate-free characterization. Recent coherent diffractive imaging with intense femtosecond X-ray pulses has verified the capability of single-shot imaging of nanoscale objects at suboptical resolutions beyond the radiation-induced damage thre...
متن کاملSingle-particle structure determination by X-ray free-electron lasers: Possibilities and challenges
Single-particle structure recovery without crystals or radiation damage is a revolutionary possibility offered by X-ray free-electron lasers, but it involves formidable experimental and data-analytical challenges. Many of these difficulties were encountered during the development of cryogenic electron microscopy of biological systems. Electron microscopy of biological entities has now reached a...
متن کاملImaging atom - clusters by hard x - ray free electron lasers
PACS. 61.10.-i – X-ray diffraction and scattering. PACS. 32.80.-t – Photon interactions with atoms. PACS. 36.40.-c – Atomic and molecular clusters. Abstract. – The ingenious idea of single molecule imaging by hard x-ray Free Electron Laser (X-FEL) pulses was recently proposed by Neutze et al. [1]. However, in their numerical modelling of the Coulomb explosion several interactions were neglected...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Structural Dynamics
سال: 2020
ISSN: 2329-7778
DOI: 10.1063/1.5144516